Coding

Part:BBa_K3003022:Design

Designed by: Ömer Can Ergül   Group: iGEM19_Bilkent-UNAMBG   (2019-10-16)


Single Chain Insulin-57 with a linker peptide (Rajpal 27)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed specifically considering the compatibility of chains with the linker sequences.

Source

Synthesized by IDT.

References

Rajpal, G., Liu, M., Zhang, Y., & Arvan, P. (2009). Single-chain insulins as receptor agonists. Molecular endocrinology (Baltimore, Md.), 23(5), 679–688. doi:10.1210/me.2008-0349

Hua, Q. X., Nakagawa, S. H., Jia, W., Huang, K., Phillips, N. B., Hu, S. Q., & Weiss, M. A. (2008). Design of an active ultrastable single-chain insulin analog: synthesis, structure, and therapeutic implications. The Journal of biological chemistry, 283(21), 14703–14716. doi:10.1074/jbc.M800313200